ASTM E1045-00 (2022) PDF

Full title and description

Designation: ASTM E1045-00 (Reapproved 2022) — Standard Specification for Pipet, Sahli Hemoglobin. This specification defines the construction, markings, dimensional tolerances, and test methods for reusable Sahli hemoglobin pipets calibrated “to contain” 20 cmm of whole blood. It covers materials, delivery-tip geometry, graduation and identification markings, capacity deviation options, and capacity and pigmentation tests used to verify conformity.

Abstract

ASTM E1045-00(2022) specifies requirements for reusable Sahli hemoglobin pipets made from spirit-bore white-back tubing (or clear glass with a white stripe). Pipets are required to be one-piece, straight, and have a gradual or concave tapered delivery tip of defined length. Required markings include a single graduation line at 20 cmm, volumetric designation, manufacturer identification and a marked capacity-deviation class. Test procedures described include capacity (gravimetric) testing, capacity-deviation verification, pigmentation (marking durability) testing, and strain-free/visual inspection criteria.

General information

• Status: Reapproved (current edition recorded as E1045-00 reapproved 2022).
• Publication date: Current edition approved Jan 1, 2022; published Feb 2022 (reapproval notice 2022).
• Publisher: ASTM International.
• ICS / categories: 11.100.30 (Analysis of blood and urine); under ASTM Committee E41 (Laboratory Apparatus), Subcommittee E41.01 (Laboratory Ware and Supplies).
• Edition / version: E1045-00 (reapproved 2022) — sometimes shown as E1045-00R22 in sales records.
• Number of pages: 3 pages.

Scope

This specification covers reusable pipets calibrated “to contain” 20 cmm of whole blood and intended for hemoglobin determinations. The standard mandates SI units, defines acceptable materials and appearance, prescribes tip geometry and graduation placement, and sets out the tests and acceptance limits for capacity and marking durability. It is intended for laboratory-use pipets manufactured and verified to meet the stated tolerances.

Key topics and requirements

• Design and shape: straight, one-piece pipet with circular cross section in transverse planes.
• Materials: spirit-bore white-back tubing or clear glass with a white stripe applied to the outer surface.
• Delivery-tip geometry: gradual or concave taper; tip length specified (typically 10–25 mm) and tip end to be ground and polished.
• Graduation and markings: single graduation line located 20 cmm from tip, volumetric designation (20 CMM), manufacturer identification, and capacity-deviation class permanently fused on the pipet.
• Capacity deviation: pipets are made with selectable maximum capacity deviation classes (historically documented as ±1.0% or ±2.0%); the selected class must be marked and the pipet must meet that class when tested.
• Testing methods: gravimetric capacity test using distilled water (high-sensitivity balance), capacity-deviation verification, pigmentation (marking durability) test, and visual/strain inspection.
• Jurisdiction: issued under ASTM Committee E41 (Laboratory Apparatus), responsibility of Subcommittee E41.01 (Laboratory Ware and Supplies).

Typical use and users

Used in clinical and laboratory settings for manual Sahli-method hemoglobin determinations and for training, quality-control, or low-resource testing scenarios where the Sahli technique is applied. Typical users include clinical laboratory technicians, manufacturers of laboratory glassware, quality and compliance officers in clinical consumables procurement, and laboratories that require small-volume calibrated pipets for hemoglobin assays.

Related standards

The standard references and relates to other ASTM specifications and practices for laboratory apparatus and packaging, such as ASTM E920 (Commercially Packaged Laboratory Apparatus), E921 (Export Packaged Laboratory Apparatus), E1133 (Performance Testing of Packaged Laboratory Apparatus), and E1157 (Sampling and Testing of Reusable Laboratory Glassware). These referenced documents support testing, packaging, and sampling practices for laboratory glassware and apparatus used alongside Sahli pipets.

Keywords

Sahli pipet, Sahli hemoglobin, pipet, laboratory glassware, 20 cmm, “to contain”, pipet graduation, capacity deviation, volumetric designation, ASTM E1045, Committee E41.

FAQ

Q: What is this standard?

A: ASTM E1045-00(2022) is a specification that defines the requirements, marking, and test methods for reusable Sahli hemoglobin pipets calibrated to contain 20 cmm of whole blood.

Q: What does it cover?

A: It covers pipet materials and construction, delivery-tip geometry, graduation and identification markings, capacity-deviation classes, and the gravimetric and pigmentation tests used to verify capacity and marking durability.

Q: Who typically uses it?

A: Clinical laboratory technicians, manufacturers and quality managers of laboratory glassware, procurement/specification engineers for clinical supplies, and labs using the Sahli hemoglobin method.

Q: Is it current or superseded?

A: The designation is E1045-00 with a reapproval noted in 2022 (often shown as E1045-00 (Reapproved 2022) or E1045-00R22). Users should confirm the current status and any later revisions directly with ASTM International before procurement or formal specification to ensure no newer revision exists after 2022.

Q: Is it part of a series?

A: It is part of ASTM’s laboratory-apparatus portfolio under Committee E41 and cross-references related specifications and practices for laboratory ware, packaging, and sampling (see related standards listed above).

Q: What are the key keywords?

A: Sahli pipet, hemoglobin, 20 cmm, pipet graduation, volumetric designation, capacity deviation, laboratory glassware, ASTM E1045.