ASTM E1193-20 PDF

Full title and description

ASTM E1193-20 — Standard Guide for Conducting Daphnia magna Life‑Cycle Toxicity Tests. This guide describes laboratory procedures for conducting chronic life‑cycle toxicity tests with the freshwater cladoceran Daphnia magna using renewal or flow‑through techniques to assess adverse effects of test materials on survival, growth and reproduction over a life‑cycle.

Abstract

E1193‑20 provides recommended methods and quality/acceptability criteria for life‑cycle toxicity testing with Daphnia magna. It covers test design, organism care, exposure techniques (renewal and flow‑through), environmental and analytical monitoring, data collection and reporting, and offers appendices with statistical and species‑specific guidance. The guide is intended to produce laboratory data suitable for chronic hazard assessment of chemicals, effluents and other aqueous test materials.

General information

• Status: Published / Active
• Publication date: December 1, 2020 (designation E1193‑20)
• Publisher: ASTM International
• ICS / categories: 07.100.20 (Microbiology of water); 13.020.30 (Environmental impact assessment)
• Edition / version: E1193‑20 (2020)
• Number of pages: 21

Scope

This guide covers procedures for obtaining laboratory data on the adverse effects of a test material (added to dilution water, not to food) on Daphnia magna during continuous exposure throughout a life‑cycle, using renewal or flow‑through techniques. It is applicable to individual chemicals, formulations, complex mixtures (for example effluents, leachates and surface waters) and certain physical factors where appropriate modifications are made. The guide also provides practical requirements for test organism sourcing and care, environmental control (temperature, dissolved oxygen, pH), test concentrations and analytical verification, acceptability criteria, and reporting.

Key topics and requirements

• Test organism: Daphnia magna — specifications for age, source, brood stock and quality control.
• Exposure techniques: guidance on selection and implementation of renewal and flow‑through systems.
• Environmental conditions: temperature control, dissolved oxygen, photoperiod, loading and water quality monitoring.
• Test material handling: stock solutions, preparation, concentration selection and analytical confirmation when required.
• Feeding, care and maintenance of test animals throughout the life‑cycle test and recommendations for diet and brood handling.
• Biological and ancillary measurements: endpoints (survival, growth, reproduction), sampling frequency and supplementary measurements (water chemistry, DO, pH).
• Acceptability criteria: control performance limits, variability limits and criteria for valid tests.
• Data analysis and reporting: calculation of chronic endpoints, statistical guidance and recommended report elements.
• Appendices: statistical guidance, diet/food recommendations and notes on applying the guide to related species (for example Daphnia pulex).

Typical use and users

Used by environmental and ecotoxicology laboratories, contract testing facilities, regulatory agencies, chemical and product manufacturers, environmental consultancies and academic researchers to generate chronic toxicity data for hazard identification, risk assessment, regulatory submissions and product stewardship. Typical applications include chronic toxicity testing of industrial chemicals, pesticides, effluents, surface waters and sediment porewaters (with appropriate modifications).

Related standards

Standards and guidance commonly used in conjunction with or referenced by E1193 include OECD Test Guidelines (for example OECD 211 — Daphnia magna Reproduction Test and OECD 202 — Acute Immobilisation Test), ISO standards for Daphnia testing (ISO 6341, ISO 10706), and ASTM guides in the E‑series dealing with aquatic toxicity such as ASTM E1192 (acute toxicity testing of aqueous samples and effluents) and ASTM E729 (acute toxicity test guidance). Users commonly consult these documents for complementary procedures, acute endpoints and international harmonization.

Keywords

Daphnia magna; life‑cycle test; chronic toxicity; aquatic toxicology; renewal; flow‑through; reproduction; survival; growth; ecotoxicology; ASTM E1193; invertebrate test; OECD 211; ISO 6341; effluent testing.

FAQ

Q: What is this standard?

A: ASTM E1193‑20 is a Standard Guide that provides recommended procedures for conducting life‑cycle (chronic) toxicity tests with the freshwater crustacean Daphnia magna to assess effects of test materials on survival, growth and reproduction.

Q: What does it cover?

A: It covers test design (renewal and flow‑through), organism sourcing and care, environmental control (temperature, dissolved oxygen, pH), preparation of test concentrations, analytical verification, acceptability criteria, data analysis and reporting. It also includes appendices with statistical guidance and species‑specific notes.

Q: Who typically uses it?

A: Ecotoxicology and environmental testing laboratories, regulatory bodies, chemical manufacturers, environmental consultants and academic researchers use it to produce chronic toxicity data for risk assessment, regulatory submissions and product/environmental monitoring.

Q: Is it current or superseded?

A: The 2020 edition (E1193‑20) is the current published edition. It updates earlier versions of E1193 (previously published as E1193 with earlier edition numbers and reapprovals).

Q: Is it part of a series?

A: Yes. E1193 is part of ASTM’s environmental assessment and biological effects guides (Committee E50 and subcommittee E50.47). It is commonly used alongside other ASTM E‑series guides for aquatic toxicity (for example E1192 and E729) and international test guidelines (OECD and ISO) addressing Daphnia and aquatic organisms.

Q: What are the key keywords?

A: Daphnia magna, life‑cycle, chronic toxicity, reproduction, flow‑through, renewal, aquatic toxicology, ecotoxicology, survival, growth, ASTM E1193.