ISO 22119-2011 PDF
Name in English:
St ISO 22119-2011
Name in Russian:
Ст ISO 22119-2011
Original standard ISO 22119-2011 in PDF full version. Additional info + preview on request
Full title and description
Microbiology of food and animal feeding stuffs — Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens — General requirements and definitions. This International Standard defines terms and specifies minimum requirements for the amplification and detection of nucleic acid sequences (DNA or RNA after reverse transcription) by real‑time PCR applied to food, feed and related environmental samples.
Abstract
ISO 22119:2011 sets out general requirements, definitions and minimum procedural and reporting elements intended to produce comparable and reproducible real‑time PCR results for the detection of food‑borne pathogens across laboratories. It covers terminology, basic performance and quality considerations for in‑house and kit‑based assays used on foodstuffs, feed and associated environmental samples.
General information
- Status: Withdrawn (superseded / revised).
- Publication date: July 2011 (Edition 1).
- Publisher: International Organization for Standardization (ISO).
- ICS / categories: 07.100.30 — Food microbiology.
- Edition / version: Edition 1 (2011).
- Number of pages: 12 pages (original ISO publication).
Scope
ISO 22119:2011 applies to the detection of food‑borne pathogens (and isolates obtained from them) in foodstuffs, animal feeding stuffs and related environmental samples using real‑time PCR. It defines terminology and specifies minimum requirements for amplification and detection steps, and for producing comparable and reproducible results between laboratories. The document has been revised and its subject matter is now covered by ISO 22174:2024, which expands and updates the general requirements for PCR used for detection and quantification of microorganisms in the food chain.
Key topics and requirements
- Definitions and terminology for real‑time PCR assays in the food and feed context.
- Minimum requirements for nucleic acid amplification and detection (including DNA and RNA after reverse transcription).
- Basic performance characteristics and quality control measures to support comparability between laboratories.
- Guidance on sample types covered (foodstuffs, animal feeding stuffs, environmental samples) and intended scope of application.
- Foundational elements for reporting results and for validation/verification workflows (as a baseline; users should consult ISO 22174:2024 for the current detailed requirements).
Typical use and users
Used by food and feed microbiology laboratories, public‑health laboratories, regulatory bodies, method developers and manufacturers of PCR reagents and test kits as a baseline reference for terminology, minimum procedural requirements and quality elements for real‑time PCR assays targeting food‑borne pathogens. Users updating practices should refer to the current ISO 22174:2024 text for the latest, authoritative requirements.
Related standards
ISO 22119:2011 was part of a family of PCR‑related ISO documents and has been superseded by ISO 22174:2024 (Microbiology of the food chain — PCR for detection and quantification of microorganisms — General requirements and definitions). Related ISO documents to consult for method validation and laboratory practices include ISO standards on method validation (for example, ISO 16140 series) and earlier PCR guidance documents that have been consolidated or revised into ISO 22174.
Keywords
real‑time PCR, PCR, food microbiology, food‑borne pathogens, detection, nucleic acid amplification, validation, ISO 22119, ISO 22174.
FAQ
Q: What is this standard?
A: ISO 22119:2011 is an International Standard that defined terms and minimum requirements for real‑time PCR methods used to detect food‑borne pathogens in food, feed and related environmental samples. The standard has since been withdrawn and its subject matter covered by a later ISO publication.
Q: What does it cover?
A: It covered definitions, basic amplification and detection requirements for real‑time PCR (including DNA and RNA after reverse transcription), and minimum quality elements intended to support comparable and reproducible results across laboratories working on food and feed microbiology. For current, expanded requirements see ISO 22174:2024.
Q: Who typically uses it?
A: Food and feed testing laboratories, public‑health and regulatory labs, assay and kit developers, and quality managers in the food sector—anybody involved in PCR‑based detection of microorganisms from the food chain.
Q: Is it current or superseded?
A: ISO 22119:2011 is withdrawn and superseded; the current authoritative document covering PCR general requirements for the food chain is ISO 22174:2024. Users should adopt ISO 22174:2024 as the up‑to‑date reference.
Q: Is it part of a series?
A: Yes — it belonged to the suite of ISO standards on PCR and food microbiology; the topic has been consolidated and updated in ISO 22174 (and related documents such as earlier ISO 20837/20838 and method‑validation standards). Consult ISO/TC 34/SC 9 outputs for the broader series.
Q: What are the key keywords?
A: real‑time PCR, PCR, detection, food‑borne pathogens, food microbiology, nucleic acid amplification, validation, ISO 22119, ISO 22174.