ISO 7102-2024 PDF

Full title and description

St ISO 7102-2024 — Infant formula — Determination of β-galactooligosaccharides (GOS) by ultra high performance liquid chromatography (UHPLC) with fluorescence detection after pre‑column derivatization. This international standard (published jointly as IDF 257:2024) specifies a validated analytical procedure for quantifying β‑GOS in powdered and liquid infant formulas and related nutritional products.

Abstract

This document specifies a method for the determination of β‑galactooligosaccharides (GOS) in infant formula (both powder and liquid) containing approximately 0.20 g/100 g to 3.00 g/100 g GOS in the product as prepared ready for consumption. The method uses UHPLC with fluorescence detection after pre‑column derivatization and has been validated in a multi‑laboratory study across representative infant formula matrices and ready‑to‑feed products. Validation study levels and spike‑recovery experiments are reported in the standard.

General information

• Status: Published.
• Publication date: 12 August 2024 (Edition 1, 2024).
• Publisher: International Organization for Standardization (ISO); published jointly as IDF 257:2024.
• ICS / categories: 67.050 (Food products; methods of analysis).
• Edition / version: Edition 1 (2024).
• Number of pages: 19.

Scope

The standard applies to the determination of β‑galactooligosaccharides (GOS) in infant formula and related nutritional products (powder and liquid) that contain GOS in the range of about 0.20 g/100 g to 3.00 g/100 g when prepared ready for consumption. It defines sample preparation, derivatization, UHPLC separation and fluorescence detection conditions, and performance criteria demonstrated by multi‑laboratory validation. The method is intended for routine and reference testing where accurate quantification of β‑GOS is required.

Key topics and requirements

• Analyte: β‑galactooligosaccharides (GOS) in infant formula matrices.
• Analytical technique: Ultra high performance liquid chromatography (UHPLC) with fluorescence detection after pre‑column derivatization.
• Applicable sample types: Powdered infant formula (reconstituted) and ready‑to‑feed liquid formula.
• Target concentration range: Approximately 0.20–3.00 g GOS per 100 g of product prepared for consumption (with validated study levels reported in the standard).
• Validation: Method validated by multi‑laboratory study with specified recovery and repeatability performance; single‑laboratory spike‑recovery experiments reported up to 3 g/100 g.
• Use for labelling, quality control and regulatory compliance where reliable quantification of GOS is required.

Typical use and users

Primary users include food and dairy testing laboratories, infant formula and specialized nutrition manufacturers (quality control, R&D), regulatory and inspection bodies, and contract testing providers. The method is intended for routine QC and for situations requiring traceable, validated measurements (e.g., label claims, regulatory checks, dispute resolution).

Related standards

Standards and documents commonly referenced alongside ISO 7102:2024 include other ISO/IDF analytical methods for infant formula and adult nutritionals (e.g., methods for fructans, proteins and vitamin/mineral determinations). Examples: ISO 22579 (fructans in infant formula/adult nutritionals), ISO 23293 (whey protein quantification in milk‑based infant formula powders) and AOAC/AOAC SMPR documents that set performance requirements for GOS and similar analytes. These related standards provide companion methods or performance frameworks for complementary analytes.

Keywords

GOS, β‑galactooligosaccharides, infant formula, UHPLC, fluorescence detection, pre‑column derivatization, IDF 257, method validation, food analysis, dairy chemistry.

FAQ

Q: What is this standard?

A: ISO 7102:2024 (also published as IDF 257:2024) is an international standard that specifies a UHPLC‑FLD method with pre‑column derivatization for determining β‑galactooligosaccharides in infant formula and related nutritional products.

Q: What does it cover?

A: It covers sample types (reconstituted powders and ready‑to‑feed liquids), the analytical procedure (derivatization, UHPLC separation and fluorescence detection), validation results from collaborative studies, and performance criteria for reliable quantification within the stated concentration range (≈0.20–3.00 g/100 g).

Q: Who typically uses it?

A: Food and dairy testing laboratories, infant formula manufacturers (QC and R&D), regulatory agencies and contract testing labs—any organization needing validated, traceable measurements of GOS for labeling, compliance or product development.

Q: Is it current or superseded?

A: ISO 7102:2024 was published on 12 August 2024 and is the current published edition (Edition 1, 2024). Users should check national catalogues or ISO announcements for any future revisions or confirmations.

Q: Is it part of a series?

A: Yes — it forms part of the body of ISO/IDF standards covering analytical methods for milk, milk products, infant formula and adult nutritionals developed under ISO/TC 34/SC 5. Companion standards address other analytes (e.g., fructans, proteins, vitamins and minerals).

Q: What are the key keywords?

A: β‑galactooligosaccharides (GOS), infant formula, UHPLC, fluorescence detection, pre‑column derivatization, IDF 257, method validation, food analysis.