DIN EN ISO 13366-1 2008-06 PDF

Full title and description

STB DIN EN ISO 13366-1 2008-06 — Milk — Enumeration of somatic cells — Part 1: Microscopic method (Reference method). Specifies a microscopic (reference) method for counting somatic cells in raw and chemically preserved cow's milk; used to prepare reference test samples and to calibrate mechanized and automated cell-counting methods.

Abstract

This standard (ISO 13366-1|IDF 148-1:2008) defines a laboratory reference procedure using microscopic examination to enumerate somatic cells in milk. It is applicable to cows' milk under specified prerequisites and is intended for establishing reference values and calibrating automated counters.

General information

• Status: Published (international standard; nationally adopted as DIN EN ISO 13366-1:2008-06).
• Publication date: ISO publication: February 2008 (Edition 2, 2008); DIN national publication/adoption: June 2008 (2008-06).
• Publisher: International: ISO (ISO/IDF); national adoption/publisher (German version): DIN (Deutsches Institut für Normung).
• ICS / categories: Milk and processed milk products (67.100.10); Food microbiology / related categories also referenced in national listings.
• Edition / version: Edition 2 (2008). Technical corrigendum ISO 13366-1:2008/Cor 1:2009 issued.
• Number of pages: ISO official edition: 17 pages; DIN national (German) publication includes national front matter and is listed as 24 pages.

Scope

Specifies a microscopic reference method for enumerating somatic cells in milk (primarily cows' milk), applicable to both raw and chemically preserved samples when prerequisites are met. The method is intended for use as a reference for preparing standard test samples and determining values to calibrate mechanized and automated somatic-cell counters.

Key topics and requirements

• Microscopic preparation and staining procedures for somatic-cell smears and slides.
• Detailed counting procedure and microscope settings required for the reference method.
• Requirements for sample collection, preservation (chemical preservation options), and handling to ensure valid counts.
• Calculation and reporting conventions for somatic cell counts (units, rounding, expression of results).
• Use of the method to produce reference values for calibration and performance verification of automated/mechanized counters.
• Normative references and corrigendum information addressing technical corrections.

Typical use and users

Used by national and commercial dairy testing laboratories, quality-control departments in the dairy industry, regulatory and inspection bodies, and manufacturers of somatic-cell counting equipment for calibration and validation of automated systems. The method is primarily applied where a validated reference (microscopic) count is required.

Related standards

Part of the ISO 13366 series on somatic-cell enumeration. Related documents include ISO 13366-2 (guidance for fluoro-optoelectronic counters / electronic particle counters) and other parts of the series; a technical corrigendum to Part 1 was published in 2009. National adoptions (DIN/EN/ISO) and regional variants may exist.

Keywords

somatic cell count; milk testing; microscopic method; reference method; ISO 13366-1; IDF 148-1; dairy quality; calibration; automated cell counter.

FAQ

Q: What is this standard?

A: ISO 13366-1:2008 (adopted as DIN EN ISO 13366-1:2008-06) is the international reference method that specifies a microscopic procedure for enumerating somatic cells in milk.

Q: What does it cover?

A: It covers sample preparation, microscopic counting procedures, sample preservation, calculation and reporting of somatic cell counts, and use of the method to establish reference values for calibrating automated counters.

Q: Who typically uses it?

A: Dairy testing laboratories, dairy industry quality-control personnel, regulatory laboratories, and manufacturers of somatic-cell-counting instrumentation for calibration and verification purposes.

Q: Is it current or superseded?

A: The 2008 edition is the current published edition (Edition 2, 2008) and a technical corrigendum was issued in 2009; ISO has later work items to revise/replace the part (committee draft activity has been registered). Users should check for any more recent revisions or replacements before relying on the text for regulatory purposes.

Q: Is it part of a series?

A: Yes — it is Part 1 of the ISO 13366 series (IDF 148) on somatic-cell enumeration in milk; other parts provide guidance on electronic/fluoro-optoelectronic counting methods and related topics.

Q: What are the key keywords?

A: Somatic cell count, milk, microscopic reference method, calibration, IDF 148, ISO 13366-1, dairy testing.