ISO 5061-2002 PDF

Full title and description

Animal feeding stuffs — Determination of castor oil seed husks — Microscope method. This International Standard specifies a laboratory procedure for isolating, identifying and quantifying husk fragments from Ricinus communis (castor oil seed) in single and compound animal feeding stuffs and in oilseed residues using chemical digestion followed by microscopic examination and weighing of residues.

Abstract

ISO 5061:2002 (2nd edition) defines a microscope-based method to detect and measure the presence of castor oil seed husks in feed materials. The method uses successive boiling with dilute nitric acid and sodium hydroxide to remove organic matrix, decantation and filtration, followed by stereomicroscopic and microscope identification of husk fragments and gravimetric determination. The method reports results in mg/kg and the published limit of detection is given as 5 mg/kg. The final identification step requires an analyst experienced in microscopic anatomy of Ricinus communis husks.

General information

• Status: Published (international standard; second edition published 2002).
• Publication date: 16 May 2002 (2002).
• Publisher: International Organization for Standardization (ISO).
• ICS / categories: 65.120 — Animal feeding stuffs.
• Edition / version: 2nd edition (2002), cancels and replaces ISO 5061:1983 (minor revision).
• Number of pages: 7 pages (published technical text as issued in 2002).

Scope

Applies to the determination of castor oil seed (Ricinus communis) husks in single and compound animal feeding stuffs and in oilseed residues. The method is intended to identify and quantify husk fragments present in feed matrices where such contamination is a concern for animal health or feed safety; it is a laboratory reference method requiring standard laboratory glassware, digestion reagents and microscopic equipment for identification. Final microscopical identification requires trained personnel.

Key topics and requirements

• Principle: successive chemical digestion (dilute nitric acid then sodium hydroxide), washing, decantation/filtration, microscopic identification and weighing of husk residues.
• Detection limit: reported around 5 mg/kg for the published method (laboratory performance may vary with matrix and analyst skill).
• Sample handling: representative test portions (typical test portion ≈ 100 g) with repeated determinations recommended to obtain an arithmetic mean.
• Equipment and materials: stereomicroscope/binocular lens (×5–×50), higher-power microscope for final identification, nylon gauze (≈100 μm), 3 mm sieve, oven (103 °C ± 2 °C), analytical balance and chemical reagents.
• Personnel: final microscopical identification requires a specialist experienced in Ricinus communis husk anatomy and microscopic techniques.
• Reporting: results expressed in mg/kg with details of sample, number of determinations and any correction factors or losses noted.

(Key technical items above are taken from the published method specification.)

Typical use and users

Used by feed-testing laboratories, quality-control departments of feed and oilseed processors, regulatory agencies and research laboratories concerned with feed safety and contamination control. Typical users are laboratory analysts trained in microscopy, feed chemists and compliance officers verifying that feed consignments meet safety requirements for prohibited or harmful seed residues.

Related standards

Related methods and guidance include general standards on sampling and sample preparation for animal feeding stuffs (for example ISO methods and regional/adopted standards that cover sample preparation and related analytical determinations). National bodies and regional catalogues list complementary methods for feed analysis and for determination of other contaminants or constituents in animal feed.

Keywords

castor oil seed, Ricinus communis, husk, animal feeding stuffs, feed analysis, microscope method, detection limit, sample preparation, feed safety.

FAQ

Q: What is this standard?

A: ISO 5061:2002 is an International Standard that specifies a microscope-based laboratory method for detecting and quantifying castor oil seed husks in animal feeding stuffs (second edition, published 2002).

Q: What does it cover?

A: It covers the analytical procedure (chemical digestion, separation, microscopic identification and weighing) used to isolate and quantify husk fragments of Ricinus communis in feed matrices, and gives performance and reporting guidance including a stated detection level.

Q: Who typically uses it?

A: Accredited feed and analytical laboratories, feed manufacturers’ quality-control teams, regulatory agencies and researchers working on feed safety and contamination monitoring. Analysts performing the method should be skilled in microscopy and identification of plant seed parts.

Q: Is it current or superseded?

A: ISO 5061:2002 is published as the second edition (2002) replacing the 1983 edition. Sources consulted list it as the 2002 edition and do not indicate a numbered replacement or withdrawal as of the available bibliographic records; users should verify with their national standards body or the ISO catalogue for any changes after 2002.

Q: Is it part of a series?

A: It was prepared by ISO/TC 34 (Food products), subcommittee SC 10 (Animal feeding stuffs) and sits among other ISO methods for feed sampling and analysis; it is a stand‑alone method specific to castor oil seed husks but is related by subject area to other ISO feed analysis standards.

Q: What are the key keywords?

A: Castor oil seed, Ricinus communis, husk, feed contamination, microscope method, animal feeding stuffs, detection limit, sample preparation.