BS ISO 15141-2018 PDF

Full title and description

STB BS ISO 15141-2018 — Cereals and cereal products — Determination of ochratoxin A — High performance liquid chromatographic method with immunoaffinity column cleanup and fluorescence detection. This document specifies an HPLC method using immunoaffinity column cleanup and fluorescence detection for the quantification of ochratoxin A in cereals and cereal products.

Abstract

This standard describes sample preparation, cleanup on an immunoaffinity column and HPLC separation with fluorescence detection for ochratoxin A in cereal matrices. The method is intended to achieve a limit of quantification of 0.2 µg/kg; the method detection limit depends on the sample matrix and the instrument used.

General information

• Status: Published (international standard confirmed; adopted in national catalogues including as BS ISO 15141:2018 and available under national prefixes such as STB).
• Publication date: 24 July 2018 (ISO edition).
• Publisher: International Organization for Standardization (ISO). Also published/adopted as BS ISO 15141:2018 by the British Standards Institution (BSI); STB denotes national adoption in Belarusian national catalogues.
• ICS / categories: 67.060 (Cereals, pulses and derived products).
• Edition / version: Edition 1, 2018.
• Number of pages: ISO edition: 12 pages; national/BS formatted publications may differ (BS ISO PDF listings indicate a longer pagination for the BSI publication).

Scope

Applies to the determination of ochratoxin A in cereals and cereal products using a high performance liquid chromatographic technique with immunoaffinity column cleanup and fluorescence detection. The procedure covers extraction, cleanup, chromatographic separation and quantification; it is intended for routine control and regulatory purposes where the target LOQ is 0.2 µg/kg (matrix- and instrument-dependent detection limits apply).

Key topics and requirements

• Target analyte: ochratoxin A (OTA) in cereals and derived products.
• Sample extraction and cleanup using immunoaffinity columns to reduce matrix interferences.
• High performance liquid chromatography (HPLC) separation with fluorescence detection for OTA quantification.
• Method performance: limit of quantification 0.2 µg/kg; method detection limit varies with matrix and instrumentation.
• Intended use for monitoring, compliance testing and laboratory control in food safety programs.
• Revision history: replaces earlier parts ISO 15141‑1 and ISO 15141‑2 (1998 editions).

Typical use and users

Used by food testing laboratories, cereal processors, grain traders, regulatory agencies and research institutions for routine monitoring, official control and validation studies of ochratoxin A in cereals and cereal products. Laboratories performing compliance testing under national or international food-safety frameworks will rely on this method or validated equivalents.

Related standards

ISO 15141 replaces and consolidates earlier parts ISO 15141‑1:1998 and ISO 15141‑2:1998 which have been withdrawn; related work is carried out under ISO/TC 34/SC 4 (Food products — methods of sampling and analysis). National adoptions (for example BS ISO 15141:2018) and other mycotoxin-specific standards and methods used for complementary analyses may be relevant for a complete testing program.

Keywords

ochratoxin A; OTA; cereals; cereal products; HPLC; immunoaffinity column; fluorescence detection; mycotoxin; food safety; ISO 15141; STB; BS ISO.

FAQ

Q: What is this standard?

A: An ISO international standard (ISO 15141:2018) that specifies an HPLC method with immunoaffinity column cleanup and fluorescence detection for determining ochratoxin A in cereals and cereal products; it is also published/adopted under national designations such as BS ISO 15141:2018 and may appear in national catalogues with prefixes like STB.

Q: What does it cover?

A: Covers extraction, immunoaffinity cleanup, chromatographic separation and fluorescence detection for quantifying ochratoxin A, with a stated limit of quantification of 0.2 µg/kg (and detection limits that depend on matrix and instrumentation).

Q: Who typically uses it?

A: Food and grain testing laboratories, cereal processors, regulators and researchers involved in mycotoxin monitoring, compliance testing and method validation.

Q: Is it current or superseded?

A: ISO 15141:2018 is the current consolidated international standard (published 24 July 2018) and it supersedes the earlier parts ISO 15141‑1:1998 and ISO 15141‑2:1998 which were withdrawn. The ISO record shows the 2018 edition as published and subsequently confirmed.

Q: Is it part of a series?

A: Historically ISO 15141 existed in multiple parts (e.g., Part 1 and Part 2, 1998); the 2018 publication consolidates the method into a single international standard under the ISO/TC 34/SC 4 committee. National adoptions (BS, STB, etc.) may exist.

Q: What are the key keywords?

A: ochratoxin A, OTA, cereals, HPLC, immunoaffinity, fluorescence detection, mycotoxin, food safety, ISO 15141.