GOST 31659-2024 PDF

Full title and description

ГОСТ 31659-2024 (ISO 6579-1:2017) — «Микробиология пищевой цепи. Горизонтальный метод обнаружения, подсчета и серотипирования бактерий рода Salmonella. Часть 1. Обнаружение Salmonella spp.» / Microbiology of the food chain — Horizontal method for the detection, enumeration and serotyping of Salmonella — Part 1: Detection of Salmonella spp. (adopted as a межгосударственный стандарт with modifications to ISO 6579-1:2017).

Abstract

This standard specifies a horizontal (matrix-independent) laboratory method for the detection of Salmonella spp. in products within the food chain, related environmental samples and primary-production specimens. It provides principles for sampling, pre-enrichment, selective enrichment, isolation on selective media, presumptive identification and confirmation steps, together with basic quality-control and biosafety requirements.

General information

• Status: Active / in force (межгосударственный стандарт adopted as a national standard of the Russian Federation).
• Publication date: Approved/issued October 11, 2024; date of mandatory introduction (date of entry into force): October 1, 2025 (with right of early application noted).
• Publisher: Adopted and introduced by the Federal Agency on Technical Regulating and Metrology (Rosstandart) as a межгосударственный стандарт (EASC context).
• ICS / categories: 07.100.30 — Food microbiology / microbiology of the food chain.
• Edition / version: 2024 (replaces the previous edition GOST 31659-2012 where indicated).
• Number of pages: 42 (typical published text length).

Scope

Applies to the detection of Salmonella spp. in foodstuffs intended for human consumption and animal feed, environmental samples taken in food production and processing areas, and samples from primary production (for example animal faeces, dust, swabs and other matrices encountered along the food chain). The document establishes the horizontal procedural framework rather than matrix‑specific validation details.

Key topics and requirements

• Horizontal (matrix‑independent) method structure: pre‑enrichment, selective enrichment, plating on selective/differential media, and confirmation of presumptive isolates.
• Specification of recommended media and reagents (including MSRV, selective broths and differential agars) and preparation notes for laboratory use.
• Incubation conditions and critical time/temperature windows (including provisions reflected in ISO 6579‑1 and its amendment concerning temperature ranges).
• Presumptive identification criteria and confirmatory biochemical / serological tests for Salmonella spp.
• Quality control, performance characteristics (sensitivity/specificity considerations), and interlaboratory verification recommendations.
• Requirements for documentation, traceability of results, and basic biosafety/handling precautions for work with Salmonella spp.
• Reference to serotyping guidance and methods to be used in conjunction with Part 3 (guidance/technical report) for full serovar determination.

Typical use and users

Used by accredited food and feed microbiology laboratories, public health and veterinary diagnostic laboratories, manufacturers’ quality control departments, regulators and conformity assessment bodies. The standard is intended to harmonize detection procedures across laboratories performing routine surveillance, food safety testing, outbreak investigation and regulatory compliance testing.

Related standards

Directly aligned with ISO 6579‑1:2017 (and its Amendment 1:2020) as the horizontal detection method; related documents include ISO/TR 6579‑3:2014 (guidance for serotyping) and earlier national/legacy versions such as GOST 31659‑2012 that this edition updates or replaces.

Keywords

Salmonella, food microbiology, detection method, horizontal method, enrichment, selective media, serotyping, food safety, ISO 6579‑1, Rosstandart.

FAQ

Q: What is this standard?

A: ГОСТ 31659-2024 is a межгосударственный (GOST) standard that specifies a horizontal laboratory method for the detection of Salmonella spp. in the food chain; it is harmonized with ISO 6579‑1:2017.

Q: What does it cover?

A: It covers the general procedural steps for detecting Salmonella in a wide range of matrices (food, feed, environmental and primary production samples), including sample pre‑treatment, enrichment, isolation, presumptive identification and confirmation, plus associated quality and biosafety considerations.

Q: Who typically uses it?

A: Accredited testing laboratories, food and feed producers’ QC labs, regulatory laboratories, public health and veterinary diagnostic services, and organizations involved in surveillance and outbreak response.

Q: Is it current or superseded?

A: It is current (active). ГОСТ 31659‑2024 was approved in October 2024 and set to enter into force on October 1, 2025 (with provisions for early application); it replaces the earlier GOST 31659‑2012 where indicated.

Q: Is it part of a series?

A: Yes — it is Part 1 of the ISO/GOST series on Salmonella methods (detection, enumeration and serotyping). Part 3 (ISO/TR 6579‑3:2014) provides serotyping guidance; other parts/technical specifications address enumeration and supplementary methods.

Q: What are the key keywords?

A: Salmonella, detection, food chain, horizontal method, enrichment, selective media, serotyping, ISO 6579‑1, food microbiology.