ISO 6579-1-2017 amd1-2020 PDF

Full title and description

ISO 6579-1:2017 — Microbiology of the food chain — Horizontal method for the detection, enumeration and serotyping of Salmonella — Part 1: Detection of Salmonella spp., with Amendment 1:2020 (ISO 6579-1:2017/Amd 1:2020) — Amendment title: "Broader range of incubation temperatures, amendment to the status of Annex D, and correction of the composition of MSRV and SC".

Abstract

ISO 6579-1:2017 specifies a horizontal culture-based method for the detection of Salmonella spp. in foods, feed, environmental samples related to food production and primary production samples. Amendment 1 (2020) updates incubation temperature ranges, corrects compositions of selective media (notably MSRV and selenite cystine medium), and changes the status of Annex D from normative to informative to clarify procedures for specific serovars such as S. Typhi and S. Paratyphi.

General information

• Status: Published; internationally adopted (standard confirmed in review process).
• Publication date: ISO 6579-1:2017 — February 2017; Amendment 1 published March 6, 2020 (Edition 2020).
• Publisher: International Organization for Standardization (ISO), prepared by ISO/TC 34/SC 9.
• ICS / categories: 07.100.30 (Microbiology of food and animal feeding stuffs).
• Edition / version: ISO 6579-1:2017 (Edition 1) with Amendment 1:2020 (ISO 6579-1:2017/Amd 1:2020).
• Number of pages: Main document — 50 pages; Amendment 1 — 11 pages (amendment document).

Scope

This part of ISO 6579 defines a horizontal method for the detection of Salmonella spp. applicable to products intended for human consumption and animal feeding, environmental samples in food production and handling areas, and primary production stage samples such as animal faeces, dust and swabs. The method is intended to detect the majority of Salmonella serovars; for some specific serovars additional or alternative culture steps are described (Annex D, now informative in the amended text).

Key topics and requirements

• Horizontal, culture-based detection workflow for Salmonella spp., including pre-enrichment, selective enrichment, plating, and confirmation steps.
• Amendment 1 (2020) extends acceptable incubation temperatures for selective and non-selective media from a tight 37 °C ±1 °C to a broader 34 °C–38 °C range (no further tolerance), increasing laboratory flexibility.
• Corrections to the composition and preparation instructions for Modified semi-solid Rappaport-Vassiliadis (MSRV) agar and selenite cystine (SC) medium to ensure correct final concentrations and improved selectivity/sensitivity.
• Change of Annex D status from normative to informative, with clarifications for detection procedures for S. Typhi and S. Paratyphi and related media formulation corrections.
• Equipment requirements clarified to ensure incubators and water baths can reliably maintain the revised temperature range and incubation performance criteria for media productivity and selectivity.

Typical use and users

Routine and accredited food microbiology laboratories, public health laboratories, official control bodies, feed and food industry quality/assurance teams, contract testing laboratories, and research organizations use ISO 6579-1:2017 together with Amendment 1:2020 for standardized Salmonella detection in regulatory testing, product release, contamination investigations and method validation/comparison.

Related standards

ISO 6579-1 is part of the broader ISO 6579 series covering detection, enumeration and serotyping of Salmonella in the food chain; regional/adopted equivalents include EN ISO 6579-1:2017/A1:2020 and national adoptions. Earlier versions (ISO 6579:2002 and its corrigenda) have been withdrawn and superseded by the 2017 edition and its amendment.

Keywords

Salmonella, detection, food microbiology, horizontal method, MSRV, selenite cystine, incubation temperature, selective media, ISO 6579-1, amendment 2020, food safety, feed testing.

FAQ

Q: What is this standard?

A: ISO 6579-1:2017 is an international standard that specifies a horizontal culture-based method for the detection of Salmonella spp. in food, feed and related environmental and primary production samples; Amendment 1:2020 updates technical details such as incubation temperatures and media compositions.

Q: What does it cover?

A: It covers the end-to-end detection workflow (pre-enrichment, selective enrichment, plating, and confirmation), the formulation and performance of key media (e.g., MSRV, RVS, MKTTn, XLD), and procedural/temperature requirements to harmonize laboratory results across different matrices.

Q: Who typically uses it?

A: Accredited food and public-health laboratories, industry quality and safety teams, contract testing services, and regulatory control bodies use this standard for routine Salmonella testing, surveillance and compliance testing.

Q: Is it current or superseded?

A: ISO 6579-1:2017 with Amendment 1:2020 is the current published version of Part 1 (the 2017 edition was confirmed in a subsequent review). Earlier ISO 6579 editions and corrigenda have been withdrawn and replaced by this edition and its amendment.

Q: Is it part of a series?

A: Yes — it is Part 1 of the ISO 6579 series, which addresses detection, enumeration and serotyping of Salmonella in the food chain; other parts and related documents address enumeration and serotyping or matrix-specific methods.

Q: What are the key keywords?

A: Salmonella, detection, food safety, horizontal method, MSRV, selenite cystine, incubation temperature, ISO 6579-1:2017/Amd 1:2020.