DIN EN ISO 14565 2000-03 PDF

Full title and description

STB / DIN EN ISO 14565 (ISO 14565:2000) — "Animal feeding stuffs — Determination of vitamin A content — Method using high-performance liquid chromatography". This document specifies an HPLC‑based analytical procedure for determination of total vitamin A (retinol, including all‑trans and cis‑isomers) in animal feeds and pet foods and is published as ISO 14565:2000; it has been adopted in various national/European versions (EN/ DIN / STN etc.).

Abstract

This standard defines a laboratory method using high‑performance liquid chromatography (HPLC) for extraction, separation and quantification of vitamin A (retinol) in animal feedingstuffs and pet foods. Results are expressed in international units per kilogram (IU/kg). The core ISO edition was published in 2000 and has been confirmed in subsequent systematic reviews; national/adopted editions (EN, DIN, STN and others) provide official translations and national forewords.

General information

• Status: Published; international standard (ISO 14565:2000), confirmed at review.
• Publication date: ISO edition published December 2000 (ISO 14565:2000); many national/adopted versions were published in 2001–2004 (EN/DIN/STN adoptions).
• Publisher: International Organization for Standardization (ISO); adopted versions published by national bodies (e.g., CEN/EN, DIN, STN, national standards bodies).
• ICS / categories: 65.120 — Animal feeding stuffs; feed analysis and testing.
• Edition / version: Edition 1 (ISO 14565:2000). National/adopted editions may carry local designations and dates (for example DIN EN ISO adoptions dated 2001/2004 depending on country).
• Number of pages: Core ISO document: 11 pages (ISO electronic edition). National adoptions may show slightly different page counts due to added national forewords (varies between ~11 and ~22 pages).

Scope

The standard applies to the determination of total vitamin A (retinol) in animal feeding stuffs and pet foods using HPLC. It covers sample preparation, extraction, chromatographic separation, identification and quantification of all‑trans‑retinyl alcohol and its cis‑isomers and the expression of results in IU/kg. It is intended for routine and regulatory laboratory use where accurate quantification of vitamin A in feeds is required.

Key topics and requirements

• Scope and applicability to animal feeds and pet foods (total vitamin A expressed as IU/kg).
• Sampling and sample preparation guidance to obtain representative, stable extracts for analysis.
• Extraction and cleanup steps appropriate for fat‑soluble vitamins prior to HPLC.
• HPLC method: recommended stationary phases, mobile phase considerations, detection and separation of all‑trans and cis isomers.
• Calibration, use of reference materials/standards, calculation of results and expression in IU/kg.
• Quality control and performance criteria (repeatability/precision and reporting requirements where applicable).

Typical use and users

Primary users are analytical laboratories performing feed and pet food testing, quality control departments within feed and pet food manufacturers, regulatory authorities overseeing feed composition and safety, and research laboratories studying vitamin levels in feeds. Laboratories use this standard to establish validated HPLC test methods and to ensure comparability of results across organizations.

Related standards

Related documents include other ISO and EN methods for feed analysis and methods for other fat‑soluble vitamins; national methods and AOAC procedures for vitamins in foods/feeds are commonly used as complementary or alternative methods. Note: the number "14565" is also used in unrelated CEN/EN standards (for example EN 14565 in the floor‑coverings series refers to a different subject); confirm the scope and committee when ordering or referencing a national/EN/DIN document.

Keywords

vitamin A; retinol; animal feeding stuffs; pet food; HPLC; feed analysis; IU/kg; ISO 14565; feed testing; chromatographic method.

FAQ

Q: What is this standard?

A: ISO 14565:2000 is an international standard that specifies an HPLC method to determine total vitamin A (retinol) in animal feeding stuffs and pet foods. It has been adopted in several national/European versions (EN / DIN / STN etc.).

Q: What does it cover?

A: It covers sample preparation, extraction, chromatographic separation, detection and quantification of all‑trans‑retinol and cis‑isomers and the reporting of results in international units per kilogram (IU/kg).

Q: Who typically uses it?

A: Feed‑testing laboratories, feed and pet food manufacturers (QC), regulatory testing bodies, and research labs concerned with vitamin A levels in feeds.

Q: Is it current or superseded?

A: The ISO 14565:2000 edition remains the published ISO document; it has been periodically reviewed and was confirmed in ISO systematic reviews (the ISO record shows the 2000 edition confirmed in review cycles). National/adopted versions will carry their own confirmation or revision dates. Always check the national body for the latest national adoption or revision.

Q: Is it part of a series?

A: It belongs to the feed analysis field (ICS 65.120). There are many other ISO/EN methods addressing different analytes in feeds (other vitamins, proximate composition, contaminants) — ISO 14565 is the specific procedure for vitamin A by HPLC.

Q: What are the key keywords?

A: Vitamin A; retinol; animal feed; pet food; HPLC; feed analysis; IU/kg; ISO 14565.